Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

Helle C. Ravn^b, Marianne L. Damstrup^a, Anne S. Meyer^b,

                                                                                               

Abstract

Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25 h, 66 °C, enzyme dosage 3.1%w/w.

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Keywords

• Immobilized Candida antarctica lipase B; Fish oil concentrates; Omega-3 fatty acids; Central composite design; Esterification; Transesterification