Thermal stability of Candida antarctica lipase B immobilized on macroporous acrylic resin particles in organic media

Yadagiri Poojari^, ,Stephen J. Clarson

 

                                                                                                                   

Abstract

Lipase B from Candida antarctica (CALB) has been exploited by many researchers for synthesis of a variety of organic compounds through esterification or transesterification reactions. Among the various immobilization media reported in the literature, the porous acrylic resin utilized in Novozym-435 has been widely studied. However, the thermal stability of free CALB and immobilized CALB (Novozym-435) in organic media at elevated temperatures for prolonged periods of time is largely unexplored. Here, we present the thermal stability and swelling of the Novozyme-435 in organic solvents (toluene and diphenyl ether) where the enzyme activity was quantified using an octyl laurate assay.

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Keywords

• Candida antarctica Lipase B;Thermal stability;Swelling;Organic media;Biocatalysis;Esterification; Transesterification