Production and partial characterization of extracellular glucose isomerase using thermophilic Bacillus sp. isolated from agricultural land

MohanaSundaram Sukumar^, ,Arun Jeyaseelan,Thirumalai Sivasankaran,Pennarasi Mohanraj, Prasanna Mani,Gowthamraj Sudhakar,Vimalraj Arumugam,Sasirekha Bakthavachalu,Alagulakshmi Ganeshan,Monika Susee

 

                                                                                                                   

Abstract

In this investigation, glucose isomerase was extracted from Bacillus sp., isolated from agricultural soil sample and purified further by ammonium sulfate fractionation followed by gel filtration chromatography using Sephadex G-25. The purified enzyme over crude was further subjected to partial characterization which includes the assessment of influence of pH, temperature, incubation time and metal ions on the stability and activity of glucose isomerase was observed. As a result, it was found that, glucose isomerase isolated from the thermophilic Bacillus sp. enumerated from the agricultural soil showed maximal stability at pH 8.0 and at temperature 70 °C within 35 min. It was also found that, at the minimum concentration of 5 mM Mg²⁺, Co²⁺and Mn²⁺ showed 41%–59% of enzyme activity.

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Keywords

• Extracellular glucose isomerase;Thermostable;Bacillus sp.;Fermentation;Stability